RESEARKA

Several outcome domains within the synthesis are supported by only a single source, precluding internal replication and amplifying the risk that a lone effect estimate reflects study-specific confounding. Blunted rest-activity rhythm amplitude and its association with all-cause, cardiovascular, and cancer mortality is documented solely by Xu 2022; no second independent cohort in the corpus reports the same relative-amplitude metric. When an entire mechanistic chain rests on one experiment—such as King 2025 showing that constant light accelerates amyloid-β plaque accumulation in 5xFAD mice—the generalizability to humans cannot be triangulated within the corpus. Single-trial outcomes should be flagged for readers as needing independent replication before informing clinical recommendations.

Citation Support

• source_1 Hu 2025
• source_2 Cheng 2024
• source_3 Panagiotou 2024
• source_4 Shim 2024
• source_5 Windred 2024