{"publication_id":"06888b97-d174-4ea3-8963-a9e4112d22e8","traces":[{"claim_id":"claim_1","claim":"Receipt 1:** \"The NAD+ precursor nicotinamide riboside decreases exercise performance in rats\" (2016) — Chronic 21-day NR administration at 300 mg/kg/day in young Wistar rats showed a tendency toward worse swimming performance versus saline controls.","citation_support":[],"candidate_sources":[{"study":"The NAD + precursor nicotinamide riboside decreases exercise performance in rats","year":2016,"doi":"10.1186/s12970-016-0143-x","url":"https://doi.org/10.1186/s12970-016-0143-x","population":"not extracted","intervention_or_exposure":"not extracted","comparator":"not extracted","endpoint":"not extracted","effect":"not extracted","risk_of_bias":"not appraised in public sidecar","directness":"primary","excerpt":"Background Nicotinamide adenine dinucleotide (NAD+) and its phosphorylated form (NADP+) are key molecules in ubiquitous bioenergetic and cellular signaling pathways, regulating cellular metabolism and homeostasis. Thus, supplementation with NAD+ and NADP+ precursors emerged as a promising strategy to gain many and multifaceted health benefits. In this proof-of-concept study, we sought to investigate whether chronic nicotinamide riboside administration (an NAD+ precursor) affects exercise performance.Methods Eighteen Wistar rats were equally divided in two groups that received either saline vehicle or nicotinamide riboside at a dose of 300 mg/kg body weight/day for 21 days via gavage. At the end of the 21-day administration protocol, both groups performed an incremental swimming performance test.Results The nicotinamide riboside group showed a tendency towards worse physical performance by 35 % compared to the control group at the final 10 % load (94 ± 53 s for the nicotinamide riboside group and 145 ± 59 s for the control group; P = 0.071).Conclusion Our results do not confirm the previously reported ergogenic effect of nicotinamide riboside. The potentially negative effect of nicotinamide riboside administration on physical performance may be attributed to the pleiotropic metabolic and redox properties of NAD+ and NADP+.","source_id":"source_1","support_kind":"candidate_source_row"},{"study":"Acute nicotinamide riboside supplementation improves redox homeostasis and exercise performance in old individuals: a double-blind cross-over study.","year":2020,"doi":"10.1007/s00394-019-01919-4","url":"https://doi.org/10.1007/s00394-019-01919-4","population":"not extracted","intervention_or_exposure":"not extracted","comparator":"not extracted","endpoint":"not extracted","effect":"not extracted","risk_of_bias":"not appraised in public sidecar","directness":"primary","excerpt":"Older individuals suffer from low NADH levels. We have previously shown that nicotinamide riboside [NR; a NAD(P)(H) precursor] administration impaired exercise performance in young rats. It has been suggested that supplementation of redox agents exerts ergogenic effect only in deficient individuals. We hypothesized that old individuals would more likely benefit from NR supplementation. We investigated the effect of acute NR supplementation on redox homeostasis and physical performance in young and old individuals. Twelve young and twelve old men received NR or placebo in a double-blind cross-over design. Before and 2 h after NR or placebo supplementation, blood and urine samples were collected, while physical performance (VO At rest, old individuals exhibited lower erythrocyte NAD(P)H levels, higher urine F NR supplementation increased NAD(P)H levels, decreased oxidative stress, and improved physical performance only in old subjects, substantiating that redox supplementation may be beneficial only in individuals with antioxidant deficiencies.","source_id":"source_2","support_kind":"candidate_source_row"}]},{"claim_id":"claim_2","claim":"Receipt 2:** \"Acute nicotinamide riboside supplementation improves redox homeostasis and exercise performance in old individuals: a double-blind cross-over study\" (2020) — Acute NR supplementation in older men (who exhibited lower erythrocyte NAD(P)H and higher urine F₂-isoprostanes at baseline) increased NAD(P)H levels, decreased oxidative stress, and improved VO₂-related performance, while no comparable benefit appeared in young subjects.","citation_support":[],"candidate_sources":[{"study":"The NAD + precursor nicotinamide riboside decreases exercise performance in rats","year":2016,"doi":"10.1186/s12970-016-0143-x","url":"https://doi.org/10.1186/s12970-016-0143-x","population":"not extracted","intervention_or_exposure":"not extracted","comparator":"not extracted","endpoint":"not extracted","effect":"not extracted","risk_of_bias":"not appraised in public sidecar","directness":"primary","excerpt":"Background Nicotinamide adenine dinucleotide (NAD+) and its phosphorylated form (NADP+) are key molecules in ubiquitous bioenergetic and cellular signaling pathways, regulating cellular metabolism and homeostasis. Thus, supplementation with NAD+ and NADP+ precursors emerged as a promising strategy to gain many and multifaceted health benefits. In this proof-of-concept study, we sought to investigate whether chronic nicotinamide riboside administration (an NAD+ precursor) affects exercise performance.Methods Eighteen Wistar rats were equally divided in two groups that received either saline vehicle or nicotinamide riboside at a dose of 300 mg/kg body weight/day for 21 days via gavage. At the end of the 21-day administration protocol, both groups performed an incremental swimming performance test.Results The nicotinamide riboside group showed a tendency towards worse physical performance by 35 % compared to the control group at the final 10 % load (94 ± 53 s for the nicotinamide riboside group and 145 ± 59 s for the control group; P = 0.071).Conclusion Our results do not confirm the previously reported ergogenic effect of nicotinamide riboside. The potentially negative effect of nicotinamide riboside administration on physical performance may be attributed to the pleiotropic metabolic and redox properties of NAD+ and NADP+.","source_id":"source_1","support_kind":"candidate_source_row"},{"study":"Acute nicotinamide riboside supplementation improves redox homeostasis and exercise performance in old individuals: a double-blind cross-over study.","year":2020,"doi":"10.1007/s00394-019-01919-4","url":"https://doi.org/10.1007/s00394-019-01919-4","population":"not extracted","intervention_or_exposure":"not extracted","comparator":"not extracted","endpoint":"not extracted","effect":"not extracted","risk_of_bias":"not appraised in public sidecar","directness":"primary","excerpt":"Older individuals suffer from low NADH levels. We have previously shown that nicotinamide riboside [NR; a NAD(P)(H) precursor] administration impaired exercise performance in young rats. It has been suggested that supplementation of redox agents exerts ergogenic effect only in deficient individuals. We hypothesized that old individuals would more likely benefit from NR supplementation. We investigated the effect of acute NR supplementation on redox homeostasis and physical performance in young and old individuals. Twelve young and twelve old men received NR or placebo in a double-blind cross-over design. Before and 2 h after NR or placebo supplementation, blood and urine samples were collected, while physical performance (VO At rest, old individuals exhibited lower erythrocyte NAD(P)H levels, higher urine F NR supplementation increased NAD(P)H levels, decreased oxidative stress, and improved physical performance only in old subjects, substantiating that redox supplementation may be beneficial only in individuals with antioxidant deficiencies.","source_id":"source_2","support_kind":"candidate_source_row"}]},{"claim_id":"claim_3","claim":"Why this is surprising:** Receipt 1 made plausible the expectation that chronic NR would uniformly impair exercise output, yet Receipt 2 shows the same anchor can flip toward ergogenic benefit when baseline redox status is deficient, suggesting age-dependent reversal rather than a stable directional effect.","citation_support":[],"candidate_sources":[{"study":"The NAD + precursor nicotinamide riboside decreases exercise performance in rats","year":2016,"doi":"10.1186/s12970-016-0143-x","url":"https://doi.org/10.1186/s12970-016-0143-x","population":"not extracted","intervention_or_exposure":"not extracted","comparator":"not extracted","endpoint":"not extracted","effect":"not extracted","risk_of_bias":"not appraised in public sidecar","directness":"primary","excerpt":"Background Nicotinamide adenine dinucleotide (NAD+) and its phosphorylated form (NADP+) are key molecules in ubiquitous bioenergetic and cellular signaling pathways, regulating cellular metabolism and homeostasis. Thus, supplementation with NAD+ and NADP+ precursors emerged as a promising strategy to gain many and multifaceted health benefits. In this proof-of-concept study, we sought to investigate whether chronic nicotinamide riboside administration (an NAD+ precursor) affects exercise performance.Methods Eighteen Wistar rats were equally divided in two groups that received either saline vehicle or nicotinamide riboside at a dose of 300 mg/kg body weight/day for 21 days via gavage. At the end of the 21-day administration protocol, both groups performed an incremental swimming performance test.Results The nicotinamide riboside group showed a tendency towards worse physical performance by 35 % compared to the control group at the final 10 % load (94 ± 53 s for the nicotinamide riboside group and 145 ± 59 s for the control group; P = 0.071).Conclusion Our results do not confirm the previously reported ergogenic effect of nicotinamide riboside. The potentially negative effect of nicotinamide riboside administration on physical performance may be attributed to the pleiotropic metabolic and redox properties of NAD+ and NADP+.","source_id":"source_1","support_kind":"candidate_source_row"},{"study":"Acute nicotinamide riboside supplementation improves redox homeostasis and exercise performance in old individuals: a double-blind cross-over study.","year":2020,"doi":"10.1007/s00394-019-01919-4","url":"https://doi.org/10.1007/s00394-019-01919-4","population":"not extracted","intervention_or_exposure":"not extracted","comparator":"not extracted","endpoint":"not extracted","effect":"not extracted","risk_of_bias":"not appraised in public sidecar","directness":"primary","excerpt":"Older individuals suffer from low NADH levels. We have previously shown that nicotinamide riboside [NR; a NAD(P)(H) precursor] administration impaired exercise performance in young rats. It has been suggested that supplementation of redox agents exerts ergogenic effect only in deficient individuals. We hypothesized that old individuals would more likely benefit from NR supplementation. We investigated the effect of acute NR supplementation on redox homeostasis and physical performance in young and old individuals. Twelve young and twelve old men received NR or placebo in a double-blind cross-over design. Before and 2 h after NR or placebo supplementation, blood and urine samples were collected, while physical performance (VO At rest, old individuals exhibited lower erythrocyte NAD(P)H levels, higher urine F NR supplementation increased NAD(P)H levels, decreased oxidative stress, and improved physical performance only in old subjects, substantiating that redox supplementation may be beneficial only in individuals with antioxidant deficiencies.","source_id":"source_2","support_kind":"candidate_source_row"}]}]}